Composite

Part:BBa_K4119017

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)


PthlA-FNRbindingsite-3->Bs2->Cpa fdx terminator

PthlF3（BBa_K4119015）-Bs2-Cpa fdx terminator


Document

Results:

The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.

Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None